SubtiBank SubtiBank
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2017-8-11 16:0:32025-05-31 14:55:13

_ec

description

6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates

branch migration transferase, 6-O-methylguanine-DNA methyltransferase, negative effector of [[protein|DisA]] activity, participates in the stabilization and/or processing of holliday junction intermediates, required for repair-by-recombination

locus

BSU00870

BSU_00870

geneLength

1374

1377

function

homologous recombination , control of c-di-AMP formation

homologous recombination, control of c-di-AMP formation, co-ordination of responses to replicative stress and genetic recombination

product

6-O-methylguanine-DNA methyltransferase

branch migration transferase, 6-O-methylguanine-DNA methyltransferase

outlinks

bsu

BSU00870

BSU_00870

Gene

Coordinates

106,096 → 107,472

106,096 107,472

The protein

Protein family

RadA subfamily (according to Swiss-Prot)

RecA family (together with [[protein|RecA]]) (according to UniProt)

Biological materials

Mutant

MGNA-B931 (yacJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1930 NBRP B. subtilis, Japan]

BKG1 (''[[gene|radA]]''::''spc''), available in [SW|Jörg Stülke]'s lab

BKG2 (''[[gene|radA]]-[[gene|disA]]::spc''), available in [SW|Jörg Stülke]'s lab

MGNA-B931 (yacJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1930 NBRP B. subtilis, Japan]

BKG1 (''[[gene|radA]]''::''spc''), available in [SW|Jörg Stülke]'s lab

BKG2 (''[[gene|radA]]-[[gene|disA]]::spc''), available in [SW|Jörg Stülke]'s lab

BKE00870 ([[gene|radA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAGTGTAAGACCTC, downstream forward: _UP4_CGTACTTCATTAGGAGGATA

BKK00870 ([[gene|radA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAGTGTAAGACCTC, downstream forward: _UP4_CGTACTTCATTAGGAGGATA

Biological materials

Expression vector

pGP2693: expression in ''E. coli'', with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

References

8793870, 9987115, 15317759, 23760274, 11544224, 25484163, 11810266, 28511132

Gene

Phenotypes of a mutant

reduced spore survival after infrared exposure [pubmed|28961460]

sensitive to mitomycin C and H2O2-induced DNA damage [pubmed|30877841]

impaired in transformation with chromosomal DNA [pubmed|30877841]

no transformation with chromosomal DNA in [[gene|recG]] mutant cells [pubmed|31350886]

The protein

Catalyzed reaction/ biological activity

reduces the activity of [[protein|DisA]] [pubmed|23760274]

binds ssDNA and Holliday junctions (preferentially over dsDNA) [pubmed|30877841]

promotes [[protein|RecA]]-mediated DNA strand exchange [pubmed|30877841]

ATPase and 5' --> 3' DNA helicase activity [pubmed|30877841]

unwinds forked DNA in the 5′→3′ direction [pubmed|31350886]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

N-terminal zinc finger (aa 10 ... 27) (according to UniProt)

central RecA-like ATPase domain (according to UniProt)

KNRFG motif (aa 255 ... 259) (according to UniProt)

C-terminal Lon domain (aa 354 ... 458) (according to UniProt)

The protein

Structure

[PDB|5LKM] (from Streptococcus pneumoniae, 63% identity) [pubmed|28561029]

The protein

[SW|Localization]

cytoplasm (according to UniProt)

Biological materials

Expression vectors

pGP2693: expression in ''E. coli'', with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

References

Reviews

26459995

References

Original publications

8793870, 9987115, 15317759, 23760274, 11544224, 25484163, 11810266, 28511132, 28961460, 28561029, 26845522, 30877841, 31350886