2017-8-11 16:0:32025-05-31 14:55:13
_ec
description
6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates
branch migration transferase, 6-O-methylguanine-DNA methyltransferase, negative effector of [[protein|DisA]] activity, participates in the stabilization and/or processing of holliday junction intermediates, required for repair-by-recombination
locus
BSU00870
BSU_00870
geneLength
1374
1377
function
homologous recombination , control of c-di-AMP formation
homologous recombination, control of c-di-AMP formation, co-ordination of responses to replicative stress and genetic recombination
product
6-O-methylguanine-DNA methyltransferase
branch migration transferase, 6-O-methylguanine-DNA methyltransferase
outlinks
bsu
BSU00870
BSU_00870
Gene
Coordinates
106,096 → 107,472
106,096 107,472
The protein
Protein family
RadA subfamily (according to Swiss-Prot)
RecA family (together with [[protein|RecA]]) (according to UniProt)
Biological materials
Mutant
MGNA-B931 (yacJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1930 NBRP B. subtilis, Japan]
BKG1 (''[[gene|radA]]''::''spc''), available in [SW|Jörg Stülke]'s lab
BKG2 (''[[gene|radA]]-[[gene|disA]]::spc''), available in [SW|Jörg Stülke]'s lab
MGNA-B931 (yacJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1930 NBRP B. subtilis, Japan]
BKG1 (''[[gene|radA]]''::''spc''), available in [SW|Jörg Stülke]'s lab
BKG2 (''[[gene|radA]]-[[gene|disA]]::spc''), available in [SW|Jörg Stülke]'s lab
BKE00870 ([[gene|radA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAGTGTAAGACCTC, downstream forward: _UP4_CGTACTTCATTAGGAGGATA
BKK00870 ([[gene|radA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAGTGTAAGACCTC, downstream forward: _UP4_CGTACTTCATTAGGAGGATA
Biological materials
Expression vector
pGP2693: expression in ''E. coli'', with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
References
Gene
Phenotypes of a mutant
reduced spore survival after infrared exposure [pubmed|28961460]
sensitive to mitomycin C and H2O2-induced DNA damage [pubmed|30877841]
impaired in transformation with chromosomal DNA [pubmed|30877841]
no transformation with chromosomal DNA in [[gene|recG]] mutant cells [pubmed|31350886]
The protein
Catalyzed reaction/ biological activity
reduces the activity of [[protein|DisA]] [pubmed|23760274]
binds ssDNA and Holliday junctions (preferentially over dsDNA) [pubmed|30877841]
promotes [[protein|RecA]]-mediated DNA strand exchange [pubmed|30877841]
ATPase and 5' --> 3' DNA helicase activity [pubmed|30877841]
unwinds forked DNA in the 5′→3′ direction [pubmed|31350886]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
N-terminal zinc finger (aa 10 ... 27) (according to UniProt)
central RecA-like ATPase domain (according to UniProt)
KNRFG motif (aa 255 ... 259) (according to UniProt)
C-terminal Lon domain (aa 354 ... 458) (according to UniProt)
The protein
Structure
[PDB|5LKM] (from Streptococcus pneumoniae, 63% identity) [pubmed|28561029]
The protein
[SW|Localization]
cytoplasm (according to UniProt)
Biological materials
Expression vectors
pGP2693: expression in ''E. coli'', with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original publications